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991.
J.H. Rodriguez S.B. Weller E.D. Wannaz A. Klumpp M.L. Pignata 《Ecological Indicators》2011,11(6):1673-1680
The air quality in agricultural areas close to industrial emission sources (chemical, metallurgical and cement plant) was evaluated through a biomonitoring study employing the epiphytic species Tillandsia capillaris Ruíz and Pav. f. capillaris. Plants were collected from a non-contaminated area in the province of Córdoba (reference site) and transplanted back to this site and into three industrial areas representing different emission sources of air pollutants: cement plant, chemical and metallurgical industries. Biomonitors were exposed to ambient air for four periods of 3 months each during one year (for determination of physiological parameters) and for four periods of 6 months each during two years (for determination of trace elements). In the exposure period coinciding with the winter season (i.e., the dry season) the plants showed the strongest global physiological damage, possibly due to higher air pollutant concentrations. The comparison among study areas indicates the highest values of foliar damage index at the chemical industries sites, possibly due to the emission of oxidizing pollutants. On the other hand, heavy metals and trace element concentrations (V, Fe, Co, Cu, Br, Ni, Zn and Pb) were associated mainly with metallurgical industries, although the chemical industries and the cement plant were associated with Ni, Zn and Ca accumulation in the biomonitor, respectively. Considering that these sites with high industrial activity are located close to soybean producing areas, further environmental and toxicological studies are necessary, taking into account food safety and human health. 相似文献
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993.
Dr. H. Sch. 《Plant Systematics and Evolution》1852,2(1):4-5
Ohne Zusammenfassung 相似文献
994.
995.
Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutaryl CoA reductase by measurement of coenzyme A 总被引:2,自引:0,他引:2
A new assay for 3-hydroxy-3-methylglutaryl CoA reductase (mevalonate:NADP oxidoreductase [acylating CoA], EC 1.1.1.34) is based upon the measurement of released coenzyme A (SH) during the reduction of 3-hydroxy-3-methylglutaryl CoA to mevalonate. Coenzyme A was measured in the presence of dithiothreitol, required for activity, by reaction with 5,5'-dithiobis(2-nitrobenzoic acid). Sodium arsenite forms a complex with the dithiol, but not with monothiols. Thus, reduced coenzyme A reacts instantaneously with the reagent and dithiothreitol reacts slowly. The absorbance due to the coenzyme A-5,5'-dithiobis(2-nitrobenzoic acid) reaction is determined by extrapolating the linear (dithiol) absorbance-time curve to the time of addition of the reagent. After subtraction of control absorbance (deletion of NADPH), the concentration of CoA-SH is calculated from epsilon(max) = 1.36 x 10(4) at 412 nm. The method of protein removal and reduction of sulfhydryl groups on the enzyme are critical. This method provides an immediate assay. Recovery of reduced coenzyme A was 98.7%. The assay is applicable for microsomes or purified enzyme and has an effective range of 0.5-50 nmoles of coenzyme A. It was applied to kinetic measurement of the pigeon liver microsomal enzyme reaction. The apparent K(m) value for 3-hydroxy-3-methylglutaryl CoA was 1.75 x 10(-5) M, and for NADPH the value was 6.81 x 10(-4) M. This method was compared with the dual-label method at high and low levels of activity. The data were not statistically different. 相似文献
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1000.
E Holler H Fischer C Weber H Stopper H Steger H Simek 《European journal of biochemistry》1987,163(2):397-405
Two forms of a DNA polymerase have been purified from microplasmodia of Physarum polycephalum by poly(ethyleneimine) precipitation and chromatography on DEAE-Sephacel, phosphocellulose, heparin Sepharose, hydroxyapatite, DNA-agarose, blue-Sepharose. They were separated from DNA polymerase alpha on phosphocellulose and from each other on heparin-Sepharose. Form HS1 enzyme was 30-40% pure and form HS2 enzyme 60% with regard to protein contents of the preparations. Form HS2 enzyme was generated from form HS1 enzyme on prolonged standing of enzyme preparations. The DNA polymerases were obtained as complexes of a 60-kDa protein associated with either a 135-kDa (HS1) or a 110-kDa (HS2) DNA-polymerizing polypeptide in a 1:1 molar stoichiometry. The biochemical function of the 60-kDa protein remained unknown. The complexes tended to dissociate during gradient centrifugation and during partition chromatography as well as during polyacrylamide gradient gel electrophoresis under nondenaturing conditions at high dilutions of samples. Both forms existed in plasmodia extracts, their proportions depending on several factors including those which promoted proteolysis. The DNA polymerases resembled eucaryotic DNA polymerase beta by several criteria and were functionally indistinguishable from each other. It is suggested that lower eucaryotes contain repair DNA polymerases, which are similar to those of eubacteria on a molecular mass basis. 相似文献